Figure 3.

APC mutants are hypersensitive to mad2 overexpression. Wild-type cells and APC subunit mutants (cut9–665^ts and nuc2–663^ts) were transformed with pREP3X vector and a set of mad2 overexpression constructs: pREP3X-mad2, pREP41X-mad2, and pREP81X-mad2. Transformants were streaked on EMM plates in the absence of thiamine so that three levels of mad2 overexpression were achieved via the three different strength nmt1 promoters: pREP3X-mad2, high; pREP41X-mad2, medium; and pREP81X-mad2, low (27). On each plate, wild-type transformants were streaked on the left and the mutant transformants on the right. The plates were incubated at 25°C.