Figure 1.

Generation of PLC β2-null mice. (A) Gene-targeting construct and the homologous recombination process. R1, EcoRI restriction site. (B) Western analysis of mouse tissues with a PLC-β2-specific antibody. Spleen and thymus homogenates from the wild type (+/+), heterozygous (+/−), and homozygous (−/−) mice were separated on a 7.5% polyacrylamide gel, transferred to a nitrocellulose membrane, and detected with a PLC β4-specific antibody (Santa Cruz Biotechnology). MW is a molecular weight standard of 200,000.