Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Apr;158(1):386–388. doi: 10.1128/jb.158.1.386-388.1984

New chloramphenicol resistance locus in Bacillus subtilis.

L M Anderson, T M Henkin, G H Chambliss, K F Bott
PMCID: PMC215433  PMID: 6425268

Abstract

A spontaneously occurring, noninducible, chloramphenicol-resistant mutant of Bacillus subtilis 168 has a mutation (cam-2) which maps in the ribosomal protein region of the chromosome near dal. Its presence does not confer dependence on chloramphenicol. Ribosomes of the cam-2 strain remained sensitive to chloramphenicol in in vitro protein synthesis. No chloramphenicol acetyltransferase activity could be detected.

Full text

PDF
386

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adoutte-Panvier A., Davies J. E., Gritz L. R., Littlewood B. S. Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions. Mol Gen Genet. 1980;179(2):273–282. doi: 10.1007/BF00425454. [DOI] [PubMed] [Google Scholar]
  2. Chambliss G. H., Henkin T. M., Leventhal J. M. Bacterial in vitro protein-synthesizing systems. Methods Enzymol. 1983;101:598–605. doi: 10.1016/0076-6879(83)01040-x. [DOI] [PubMed] [Google Scholar]
  3. Dedonder R. A., Lepesant J. A., Lepesant-Kejzlarová J., Billault A., Steinmetz M., Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–993. doi: 10.1128/aem.33.4.989-993.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Haldenwang W. G., Banner C. D., Ollington J. F., Losick R., Hoch J. A., O'Connor M. B., Sonenshein A. L. Mapping a cloned gene under sporulation control by inserttion of a drug resistance marker into the Bacillus subtilis chromosome. J Bacteriol. 1980 Apr;142(1):90–98. doi: 10.1128/jb.142.1.90-98.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Howard G. A., Traut R. R. Separation and radioautography of microgram quantities of ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis. FEBS Lett. 1973 Jan 15;29(2):177–180. doi: 10.1016/0014-5793(73)80555-1. [DOI] [PubMed] [Google Scholar]
  6. Osawa S., Takata R., Tanaka K., Tamaki M. Chloramphenicol resistant mutants of Bacillus subtilis. Mol Gen Genet. 1973 Dec 20;127(2):163–173. doi: 10.1007/BF00333664. [DOI] [PubMed] [Google Scholar]
  7. Shaw W. V. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 1975;43:737–755. doi: 10.1016/0076-6879(75)43141-x. [DOI] [PubMed] [Google Scholar]
  8. Wilson F. E., Hoch J. A., Bott K. Genetic mapping of a linked cluster of ribosomal ribonucleic acid genes in Bacillus subtilis. J Bacteriol. 1981 Nov;148(2):624–628. doi: 10.1128/jb.148.2.624-628.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES