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Preincubation of patient serum with protein G removed inhibitory activity. MOv-γ-transduced T cells were incubated overnight with IGROV-1tumor cells, and antitumor activity was determined by the amount of IFN-γ secreted. T cell activity was inhibited in the presence of serum taken from patient 9 afterTcell transfer. This inhibitory activity of posttreatment serum was removed by pretreatment of serum with protein G.
