Table 4.

Persistence of gene-modified T cells in blood of patients

Cycle	Patient 1		Patient 2		Patient 3		Patient 4		Patient 5		Patient 6		Patient 7
	Day/h	%	Day/h	%	Day/h	%	Day/h	%	Day/h	%	Day/h	%	Day/h	%
1			(1 h)	0.16	(1 h)	0.61	(1 h)	0.16	(1 h)	3.9	(1 h)	2.57	(1 h)	2.87
			(12 h)	0	(6 h)	0	(6 h)	0.06	(6 h)	0.24	1	0	1	0.05
			2	0	1	0	1	0	(12 h)	0	3	0.02	5	0.12
	ND		5	0.13	3	0.22	3	1.47	2	0	4	2.39	27	0.09
			14	0	6	0.5	5	0.47	3	0.3	5	2.97
			21	0	13	0	13	0	5	2.74
2			(1 h)	0.98	(1 h)	0	(1 h)	0.52			(1 h)	1
			2	0	(6 h)	0.46	1	0			(6 h)	0.08
	ND		5	0.1	1	0	5	4.24	ND		1	0	ND
			14	0	13	0.26	13	0.57			3	0
			21	0.18							5	0
											13	0
											30	0
3	(1 h)	0	2	0.14	(1 h)	0.48	(1 h)	0.89
	(12 h)	0.04	3	0.54	13	0.06	(6 h)	0.23
	1	0.27	21	0.07			1	0.08	ND		ND		ND
	2	0.8					3	<0.03
	5	0.31					5	0.05
	14	0.05					13	0
	21	0
	Patient 8		Patient 9		Patient 10		Patient 11		Patient 12		Patient 13		Patient 14
	Day/h	%	Day/h	%	Day/h	%	Day/h	%	Day/h	%	Day/h	%	Day/h	%
1	(1 h)	>1	1	3.72	1	1.86	1	6.17	1	1.52	1	2.92	1	2.75
	(6 h)	>1	2	3.66	2	2.33	6	0.61	2	0.78	2	2.9	2	2.54
	1	>1	4	0.42	12	0.23	8	1.82	8	0.04	8	0.02	8	0.09
	2	>1	7	0.6	26	0	13	1.44	14	0.01	9	0.06	15	0.06
	4	0.43	12	0.44	34	0	27	1.44	21	0.01	29	0.01	21	0.09
	13	0.04	18	0.16	55	0	39	1.36	26	0.06	39	0	28	0.07
			25	0			48	0.01	365	0.03			32	0.04
			34	0.04									48	0.02
			76	0
2	(1 h)	>1	1	2.88	1	2.03
	(6 h)	0.4	2	0.52	7	0.06
	1	>1	3	0.04	27	0	ND		ND		ND		ND
	3	0.82	8	0
	12	0	42	0
	38	0

NOTE: The quantity of neomycin phosphotransferase gene-positive T cells as a percentage of total PBMCs was determined by PCR-ELISA. Although persistence varied between patients, transferred T cells were generally present in large numbers up to day 5 following transfer but were absent or barely detectable by 2 to 3 weeks after transfer.

Abbreviation: ND, not done.