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. 2007 Feb;129(2):161–173. doi: 10.1085/jgp.200609672

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Kinetics of blockade by amiloride-related compounds. (A) Time course of the effect of benzamil on A713wt in outside-out macropatches. V _h = −60 mV. Traces corresponding to the effect of 0.1, 0.4, 1.6, and 20 μM benzamil are shown and shaded in proportion to the concentration. (B) On-rates depend on concentration, voltage, and compound. Closed circles are amiloride at V _h = −60 mV (n = 6), open circles are amiloride at V _h = −100 mV (n = 3), hatched circles are benzamil at V _h = −60 mV. (C) Off-rates depend on voltage and compound, but not concentration. All points are mean ± SEM; lines are fits to averages weighted by the standard deviations.