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. 2007 Feb;129(2):161–173. doi: 10.1085/jgp.200609672

Figure 7.

Figure 7.

Macroscopic current and amiloride block of MEC-4/MEC-10 heteromultimeric channels. (A) Amiloride-sensitive current in wild type, cysteine single-mutant, and cysteine double-mutant heteromeric channels. (B) Amiloride-sensitive current in wild type, aspartate single-mutant, and aspartate double-mutant heteromeric channels. (C) Amiloride-sensitive current in wild type, valine single-mutant, and valine-double mutant heteromeric channels. In A–C, bars are mean ± SEM for the number of cells indicated above each bar. For ease of comparison, data for wild-type heteromeric channels are repeated in A–C. *, P < 0.01, Student's t test for the indicated comparison. (D) Schematic diagram of the mutant cycle, including K i' measured at −60 mV for each combination. Values are mean ± SEM (in μM) for the number of measurements indicated in parenthesis. (E) Coupling energy, RTlnΩ, for mutations that replace the wild-type alanine with C, T, D, or V in MEC-4 and MEC-10. One-way ANOVA showed that the four K i values used to calculate each Ω differed from one another at P < 0.0005. See Table S1 for K i' values and Table S2 for full results of the ANOVA. †, data are from Goodman et al. (2002).