TABLE III.
Response Parameters of RhEQ/mG Rods for Flashes at Each Wavelength (n = 14)
| 500 nm pre | 600 nm | 652 nm | 676 nm | 500 nm post | |
|---|---|---|---|---|---|
| RhEQ: mG (%) | 90: 10 | 31: 69 | 16: 84 | 12: 88 | 90: 10 |
| Dark current (pA) | 13.0 ± 0.7 | 13.3 ± 0.6 | 13.4 ± 0.5 | 13.6 ± 0.6 | 13.6 ± 0.6 |
| Time to peak (ms) | 145 ± 4 | 150 ± 6 | 146 ± 6 | 145 ± 5 | 151 ± 9 |
| Integration time (ms) | 276 ± 18 | 233 ± 18a | 228 ± 17a | 228 ± 14a | 289 ± 18 |
| σ2/ m (pA) | 0.48 ± 0.02 | 0.40 ± 0.03a | 0.36 ± 0.03a | 0.30 ± 0.03b | 0.47 ± 0.03 |
RhEQ: mG represents the predicted percentages of E122Q rhodopsin and mouse green photoactivated at each wavelength in RhEQ/mG rods (see Results). Response parameters were obtained from 14 RhEQ/mG rods, for each of which stimuli of 500, 600, 652, 676, and 500-nm flashes were delivered in the order. The 500-nm flashes used in the first and the end of the experiment are referred as 500 nm pre and 500 nm post, respectively. The kinetics parameters of single-photon response were taken from dim flash responses, the average of which is <0.2rmax. σ2/m is the ensemble variance-to-mean ratio of dim flash stimuli (>30 times) for each wavelength. Values are means ± SEM.
Difference from 500-nm pre flashes being statistically significant, P < 0.05.
Difference from 500-nm pre flashes being statistically significant, P < 0.005. The statistical significance was determined by using the two-tailed paired Student's t test.