Table 3. Serotype specificity of antibodies induced in CD46-IFNAR mice after immunization with recombinant vectors.
DV1 | DV2 | DV3 | DV4 | |||||
Ab titera | FRNT50b | Ab titera | FRNT50b | Ab titera | FRNT50b | Ab titera | FRNT50b | |
MV | 3,000 | 160 | <100 | nd | <100 | nd | <100 | nd |
MV-EDIII | 3,000 | 160 | <100 | 40 | <100 | 40 | <100 | 40 |
MV-EDIII-ectoM | 300,000 | 10240 | 500 | 40 | <100 | 20 | <100 | 10 |
Determined by ELISA using plates coated with rEDIII from DV1, 2, 3 or 4 produced in E. coli (pooled heat-inactivated post-DV inoculation sera from experiment presented in Table 2).
FRNT50 is the highest serum dilution that reduced the number of DV focus-forming units (FFU) on Vero cells by at least 50%.