Figure 3.

Sequence analysis of randomly recombined gene libraries before (a) and after (b) screening for protease thermostability. Lines represent 986 bp of subtilisin E gene including 45 nt of its prosequence, the entire mature sequence, and 113 nt after the stop codon. Crosses indicate positions of mutations from 1E2A, and triangles indicate positions of new point mutations introduced during the DNA shuffling procedure.