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Nonlinear elasticity probed by the σ-pulse protocol. Inverse creep compliance, 1/J, as a function of applied stress pulse amplitude, σ, evaluated at delay times: t [s] = 1 (diamonds), 2 (circles), 3 (double triangles), 4 (triangles), 5 (squares) for actin concentration cA = 9.5 μM, temperature T = 21°C, and average polymer length L = 21 μm. (Inset) The time dependence of the compliance J(t) for different σ values.
