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. 2007 Dec 12;104(51):20244–20249. doi: 10.1073/pnas.0708916105

Fig. 1.

Fig. 1.

MRAP promotes MC2 receptor maturation. (A–D) CHO cells were transfected with HA-MC2 receptor (MC2R), RAMP1, MRAP, or MRAPct. (A) Cell surface receptor detected by ELISA, using anti-HA antibody on fixed nonpermeabilized cells. (B) Surface staining of live cells with anti-HA antibody. (C) cAMP response to 100 nM ACTH. (D) Western blot of immunoprecipitated HA-MC2 receptor. (E) Cells were transfected with MRAP or RAMP1 and N-terminally HA-tagged melanocortin (MC) receptors 1–5, β2-adrenergic receptor, or TRH receptor. Surface receptors were quantified as in A, and values are normalized to the RAMP1 control for each receptor. Shown are the mean and range from two experiments, each containing two or three replicates. Control expression was significantly higher for MC5, β2-adrenergic, and TRH receptors than for MC1–4 receptors. Total receptor expression, measured by ELISA after addition of detergent, was within ±40% for all receptors except the MC4 receptor, which expressed at 20% the level of other receptors. *, P < 0.05 vs. mock-transfected.