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. 2007 Dec 11;104(51):20256–20261. doi: 10.1073/pnas.0706914105

Fig. 3.

Fig. 3.

Rate of ATP synthesis at various [ATP]/[ADP][Pi] ratios. (a) The raw data of ATP synthesis reaction at each [ATP]/[ADP][Pi] ratio (A–D) summarized in Table 1. Each set of raw data was corrected for its baseline. The luminescence was calibrated by the addition of standard ATP. The different ΔpHeq values are given next to each trace. (b) ATP synthesis and hydrolysis as a function of ΔpH. Each symbol represents data at different [ATP]/[ADP][Pi] ratio conditions (A–D). Arrows indicate ΔpHeq, where the ATP synthesis rate becomes zero. The data are the average of three to seven measurements. The error limits are the standard deviations. (c) The energy contribution resulting from the [ATP]/[ADP][Pi] ratio as a function of Δμ̃H+eq given as the Eq. 3. Error bars in symbols are based on standard error values at each [ATP]/[ADP][Pi] ratio condition (see SI Table 3). The slope gives the H+/ATP ratio of n, and the y intercept gives the standard Gibbs free energy of ΔGo. The estimated n value of 4.0 ± 0.1 from four data points has a 95% confidence interval of 3.7–4.3.