Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Formation of MII and proton uptake. (A) Time course of MII formation (blue trace) and proton uptake (red trace) at 20°C and pH 6.0 monitored as absorbance changes at 380 nm (for rhodopsin) and at 595 nm (for BCP), respectively (see Materials and Methods). (Inset) UV/visible spectrum of WT rhodopsin in the dark (black trace) and after photoactivation (gray trace). After photoactivation, the MII state predominates in DM solutions. (B) The same data collected for rhodopsin 227R1.
