Fig. 4.

Fhit and β-catenin are recruited to cyclin D1, axin2, MMP-14, and survivin promoters in MCF-7 cells and regulate growth. (a) ChIP and two-step ChIP analyses were performed by using anti-Fhit and anti-β-catenin antibodies in the indicated order. IgG was used as a control. Fhit is not associated with the GAPDH promoter as a general transcription factor as shown by ChIP with anti-TFIIB. (b) Anchorage-independent growth in soft agar of MCF-7 cells transiently transfected with the indicated constructs. Numbers summarize results of four experiments.