Table 1.
Wnt signaling maintains VPC competence in the L2 stage
| Mutants | Fused/tertiary/induced,* % |
N | |||||
|---|---|---|---|---|---|---|---|
| P3.p | P4.p | P5.p | P6.p | P7.p | P8.p | ||
| arIs99† | 50/50/0 | 0/100/0 | 0/0/100 | 0/0/100 | 0/0/100 | 0/100/0 | 60 |
| arIs100† | 52/48/0 | 0/100/0 | 0/0/100 | 0/0/100 | 0/0/100 | 0/100/0 | 42 |
| cwn-1(ok546) | 98/2/0 | 87/11/0 | 11/0/89 | 0/0/100 | 0/8/92 | 2/98/0 | 110 |
| egl-20(n585ts)‡ | 96/4/0 | 54/45/0 | 3/0/97 | 0/0/100 | 0/3/97 | 1/99/0 | 75 |
| cwn-2(ok895) | 77/23/0 | 6/93/0 | 2/0/98 | 0/0/100 | 0/1/99 | 2/98/0 | 111 |
| mom-2(ne874ts)§ | 51/49/0 | 0/100/0 | 0/0/100 | 0/0/100 | 0/0/100 | 0/100/0 | 103 |
| lin-44(n1792) | 39/61/0 | 1/99/0 | 0/0/100 | 0/0/100 | 0/0/100 | 0/100/0 | 113 |
| cwn-1(ok546); egl-20(n585ts)‡ | 96/0/0 | 94/0/1 | 26/0/73 | 4/0/90 | 16/11/57 | 11/56/0 | 73 |
| mig-14(ga62) | 100/0/0 | 96/4/0 | 21/3/76 | 3/1/96 | 6/24/70 | 12/88/0 | 101 |
Wnt signaling appears to compromise the L2 fusion event: we staged mig-14(ga62) hermaphrodites by using a marker that is expressed during molting events (mlt-10::gfp)(40), and found that VPCs fuse either before or during the L2 molt (data not shown). For routine scoring of different genotypes, shown here, L3 hermaphrodites were scored for expression of GFP in P3.px-P8.px or their descendants so that the 3° or induced fates could also be scored. However, spot-checks of L1 and L2 stage worms of all genotypes indicate that fusion does not occur in the L1 stage and can occur in the L2 stage.
*The percentage occasionally does not equal 100%, because cells were missing or had other abnormalities.
†All wnt mutants, except for mom-2(ne874ts), carry the hypodermal marker arIs99[dpy-7::2Xnls::yfp]. mom-2(ne874ts) carries arIs100[dpy-7::2Xnls::yfp].
‡Grown at the nonpermissive temperature, 25°C.
§mom-2(ne874ts) was grown at 15°C, and bleached mixed-stage eggs were transferred to the nonpermissive temperature.