Fig. 3.
Effect of SgrS base pairing and SgrT on recovery from glucose-phosphate stress and growth in glucose minimal medium. (A) A ΔsgrS::kan, lacIq+ host strain (CV104) carrying plasmids (pHDB3, pLCV1, pLCV5, pBRCV7, and pBRCV8) with alleles described in Fig. 2 was grown in LB with ampicillin and IPTG. Cells were stressed by addition of 0.5% αMG at early log phase. The data shown are representative of at least three independent experiments. (B) The strains described in A were grown in minimal A medium with glucose in the presence of ampicillin and IPTG. The results shown are representative of at least three independent experiments.