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SgrT does not affect levels of ptsG mRNA. The ΔsgrS::kan, lacIq+ strain CV104 carrying constructs depicted in Fig. 2 was grown to mid-log phase in LB with ampicillin. Northern blot analysis was performed on total RNA extracts harvested at times indicated after cells were exposed to IPTG to induce expression of sgrS and sgrT constructs. The blot was probed for ptsG mRNA (indicated at the left).
