Fig. 4.

VCAM-1 transcripts in lung and protein expression on lung endothelium are reduced in sensitized, antigen-challenged CXCR2^−/− mice. (A) Relative VCAM-1 mRNA expression (compared with GAPDH) in lung from sensitized, antigen-challenged WT (Left) and sensitized, antigen-challenged CXCR2^−/− (Right) mice 1 day after challenge (n = 7, from two separate experiments). The asterisk (*) indicates statistical significance (P < 0.05), as determined by a two-tailed Student's t test. (B–E) Representative pictures (of four mice of each genotype in two separate experiments) of VCAM-1 expression on lung endothelium in sensitized WT mice (B), sensitized CXCR2^−/− mice (C), sensitized, antigen-challenged WT mice (D), and sensitized, antigen-challenged CXCR2^−/− mice (E).