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. 2007 Dec 11;104(51):20490–20495. doi: 10.1073/pnas.0710145104

Fig. 1.

Fig. 1.

DC maturation by TLR-L enhances iNKT cells basal recognition. (A and B) Human DCs were left untreated (Imm) or matured with a panel of TLR-L at the indicated concentrations in the presence or absence of the iNKT line MS mix. The means of triplicate values and standard deviation of IL-12 p40 (A) and IFN-γ (B) of one ELISA experiment representative of three are shown. IL-12 p70 was detected only in samples stimulated with R-848 (data not shown). Soluble factors, like type I IFN, may influence the overall secretion of IL-12 by DC, hence the differences between different TLR-L. (C and D) Activation of different subsets of NKT cells by TLR-L-matured DC. Four iNKT cell lines and a noninvariant Vα24-negative NKT cell line, were incubated with DCs and R-848 or LPS. The means of triplicate values and standard deviation of IFN-γ (C) and IL-12 p40 (D) of one ELISA experiment representative of three are shown. DCs from different donors were used in A and B and C and D, hence the variability in the absolute amounts of IFN-γ released by iNKT cells.