Fig. 5.
A triple miRNA mimic-encoding lentivirus effectively suppresses oncogenic activity of Bcr-Abl in vivo, whereas the shRNA against the junction region is less potent. CB17.scid/scid mice were injected with 1 × 106 Ba/F3-Fluc-p210-IRES-EYFP cells transduced with control shRNA (scrambled-2 mice) and shRNA targeted against the junction region (miRNA 4 mice) (A) or various miRNAs (scrambled-8 mice; single miRNA-8 mice; double miRNA-8 mice; and triple miRNA-10 mice) (B). Kaplan–Meier survival curves were plotted. Mice were imaged at the indicated time points by using a Xenogen IVIS 100 system to record bioluminescent images of luciferase activity indicating the presence of Ba/F3-Luc-p210-IRES-EYFP cells. A false color scale to demonstrate light intensity is shown. The scale is between 2.472e ± 0.06 and 2.472e ± 0.07 photons per s per cm2 per steridian.