Fig. 4.
sir-2.1 is required for increased thermotolerance and lifespan in H2S. (A) H2S does not increase thermotolerance of animals that have a deletion in sir-2.1. The mean survival time of sir-2.1(ok434) animals grown in H2S and exposed to high temperature in H2S (solid line) is 9.8 ± 0.3 h (n = 20), which is not significantly longer than untreated controls in room air (dashed line; mean survival 9.6 ± 0.3 h; n = 20). (B) H2S does not increase the lifespan of sir-2.1(ok434) animals. The lifespan of sir-2.1(ok434) animals raised in H2S is 20.0 ± 1.6 days (solid line; n = 47), which is statistically indistinguishable from control animals in room air (dashed line; 22.2 ± 1.2 days; n = 26). Indicated P values were determined by log-rank analysis.