Figure 1.

Isolation and cloning of full-length ATM cDNA. The strategy involved combining a partial 5.9-kb ATM cDNA (22) with an overlapping 4.7-kb PCR fragment from the 5′ end of the mRNA to generate a fragment of 9.58 kb. This cDNA was cloned into an EBV-based vector (pEBV-His-B) containing a hexahistidine tag sequence to generate pEAT11 for constitutive expression. The insert and the hexahistidine sequence from this construct was subsequently subcloned into a second EBV-vector pMEP4 (33), which contains a metallothionein II inducible promoter. This construct was designated pMAT1.