Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 May;158(2):737–738. doi: 10.1128/jb.158.2.737-738.1984

Expression of a Thiobacillus ferrooxidans origin of replication in Escherichia coli.

D E Rawlings, I Pretorius, D R Woods
PMCID: PMC215493  PMID: 6373729

Abstract

A cryptic plasmid from an autotrophically grown arsenic-resistant strain of Thiobacillus ferrooxidans was isolated and cloned into pBR325. The origin of replication of pBR325 was deleted, and the recombinant plasmid was shown to replicate in Escherichia coli, using an origin of replication located on the Thiobacillus plasmid.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolivar F. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. Gene. 1978 Oct;4(2):121–136. doi: 10.1016/0378-1119(78)90025-2. [DOI] [PubMed] [Google Scholar]
  2. Brown A. M., Willetts N. S. A physical and genetic map of the IncN plasmid R46. Plasmid. 1981 Mar;5(2):188–201. doi: 10.1016/0147-619x(81)90020-2. [DOI] [PubMed] [Google Scholar]
  3. Hedges R. W., Baumberg S. Resistance to arsenic compounds conferred by a plasmid transmissible between strains of Escherichia coli. J Bacteriol. 1973 Jul;115(1):459–460. doi: 10.1128/jb.115.1.459-460.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES