Figure 1.
Repair of UV-induced CPDs at single nucleotide resolution along the transcription initiation site of the S. cerevisiae RPB2 locus. Data are for the template DNA strand, nucleotides −40 to +200 with respect to the start site of transcription. (a) Wild-type RAD+, (b) isogenic rad7Δ, and (c) rad7Δrad26Δ cells were irradiated with 70 J/m2, and repair was allowed for 0, 20, and 40 min. The large arrow indicates the major transcription initiation site and the direction of transcription. Samples mock-treated or treated with the dimer-specific enzyme T4endoV are denoted − and +, respectively.