Figure 3.
Repair of UV-induced CPDs at single nucleotide resolution along the template (a) and the nontemplate strand (b) of the S. cerevisiae URA3 locus in a rad26rad7 strain. Cells were irradiated with 70 J/m2, and repair was allowed for 0, 20, 40, and 80 min. The large arrow indicates the major transcription initiation site and the direction of transcription. Samples mock-treated or treated with the dimer-specific enzyme T4endoV are denoted − and +, respectively. More DNA was present in b. t = 40.