Figure 4.

Graphic representation of NER for the URA3 transcribed strand in (a) rad28, (b) rad14, and (c) rad3–2 genetic background. Data for the rad28 mutant were obtained with time samples of 0, 5, 10, 15, and 20 min of incubation after irradiation whereas 0, 20-, 40-, 60-, and 120-min time samples were used for repair analysis of the rad14 and rad3–2 mutants. Repair half-time values, determined as the time at which 50% of the initial CPD signal was removed were calculated for each individual CPD position and depicted above its corresponding dipyrimidine position. Repair t½ = U indicates that CPDs were unrepaired after 2 hr of incubation.