Table 3.

Fidelity interactions between T4 mutator mutations and replicative polymerase

Mutator gene and allele*	Gene product†	rII tester mutation*	Growth temp. (°C)	No. of stocks	T4 polymerase‡			RB69 polymerase‡
					wt	ts	Ratio	wt	ts	Ratio
32tsG26	Ssb	UV183	32	10	19	40	2.1	5.8	47	8.1
			37	5	3.8	11	3.0	2.0	6.5	3.3
41tsA14	DNA	375	32	5	4.3	64	15	1.4	28	19
	helicase		37	20	6.3	26	4.1	3.9	21	5.4
44tsB110	Clamp	Ed144	32	5	9.7	33	3.4	26	39	1.5
	loader		37	10	5.8	25	4.3	8.2	15	1.9
45tsCB129	Clamp	375	32	5	4.3	60	14	1.4	28	20
			37	10	5.3	28	5.3	1.2	17	14

^*The gene 32 mutator (26), the gene 41 and 45 mutators (27), and the gene 44 mutator (28) were described previously. rUV183 is an amber mutation, r375 is an ochre, and rED144 is a frameshift. 

^†For a review of the functions of these proteins see Nossal (5). 

^‡Values in the wt (wild-type) and ts columns are median revertant frequencies per 10⁸ particles. The ratios are the ts/wt values.