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Stability analysis of PAX3 and PAX3-FKHR transcripts. SJRH28 cells were treated with 10 μg/ml actinomycin D for the indicated times. RNA was isolated, and the level of expression was determined by RNase protection in triplicate. Mock-treatment did not significantly alter RNA expression (data not shown). Results are shown as relative test (PAX3, open box; PAX3-FKHR, closed diamond) to GAPDH ratios.
