Figure 2.

Internalization of peptides in RMA/S cells. RMA/S cells were treated with 20 units of γIFN for 20 h and 1 μg/ml brefeldin A (BFA) for 3 h before peptide addition. Cells were then incubated with either the SIINFEKL peptide or no peptide for an additional 2 h. Cells were then fixed, permeabilized, and stained with the 25-D1.16 antibody detected with Texas Red conjugated donkey anti-mouse IgG (Right). Cells were coincidentally stained with fluorescein-conjugated Con A (Left).