Figure 3.

Cytofluorographic analysis of K^dFL binding to VV-infected L cells. The binding of K^dFL1 and K^dFL2 to L cells infected with rVVs expressing K^d, ER-retained K^d, or influenza virus hemagglutinin in the presence or absence of the indicated competitor peptides was determined by cytofluorography. The expression of K^d by rVV-infected cells also was monitored by indirect immunofluorescence using the anti-K^d mAb SF1.1.1. (42). Cell surface expression was determined using live cells. To enable detection of intracellular K^d, cells were fixed and permeabilized. This results in the loss of surface K^d, accounting for the decrease in mAb binding to VV-K^d-infected cells (13).