Table 1.
Efficiencies of CD8+ CD44low LN T cell cloning and cytokine mRNA detection among subclones
| Parameter | Experiment 1 | Experiment 2 | Experiment 3 | Experiment 4 | ||||
|---|---|---|---|---|---|---|---|---|
| Primary cultures | ||||||||
| No. parent cells cultured | 480 | 480 | 180 | 300 | ||||
| % cultures with ≥1 cell at day 2 | 72% | 75% | 59% | 58% | ||||
| % cultures with ≥2 cells at day 2 | 69% | 62% | 46% | 52% | ||||
| Secondary cultures | IL-2 | IL-2 + IL-4 | IL-2 | IL-2 + IL-4 | IL-2 | IL-2 + IL-4 | IL-2 | IL-2 + IL-4 |
| No. cells transferred on day 2 | 78 | 75 | 58 | 56 | 68 | 60 | 58 | 51 |
| % cells that recloned by day 5 | 83% | 75% | 95% | 88% | 85% | 75% | 88% | 84% |
| Mean subclone size on day 5 | 52 ± 38 | 38 ± 32 | 66 ± 49 | 43 ± 40 | 106 ± 70 | 86 ± 69 | 130 ± 63 | 106 ± 54 |
| No. subclones assayed by RT-PCR | 55 (38) | 54 (38) | 44 (37) | 44 (37) | 42 (38) | 44 (38) | 30 (30) | 30 (30) |
| No. β-actin/CD3ɛ+ subclones | 37 (26) | 35 (26) | 25 (25) | 20 (18) | 42 (38) | 44 (38) | 30 (30) | 30 (30) |
| No. informative parent cells | 20 | 13 | 38 | 30 | ||||
Numbers in parentheses indicate the number of parent cells from which the assayed subclones were derived.