Table 2.
Cytokines considered | No. divisions before cell transfer | No. parent cells assayed | No. multipotential parent cells |
---|---|---|---|
IL-4 | 1 | 70 | 32 (46%) |
2 | 31 | 19 (61%) | |
Total 101 | 51 (50%) | ||
6 cytokines | 1 | 70 | 60 (86%) |
2 | 31 | 30 (97%) | |
Total 101 | 90 (89%) |
Parent cells were defined as multipotential if two of their subclones expressed different cytokine combinations. Frequencies were estimated considering expression of IL-4 alone, or all six assayed cytokines (IFN-γ, IL-2, IL-4, IL-5, IL-6, and IL-10), and for parent cells whose progeny were separated after either one or two divisions.