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. 1997 Jul 22;94(15):8076–8081. doi: 10.1073/pnas.94.15.8076

Table 1.

Ku70-deficient ES cells fail to support both signal and coding joining in V(D)J recombination

Cell line	pJH200 (signal joining)				pJH290 (coding joining)
Cell line	(Amp^r + Cam^r)/Amp^r	%	Relative level	Fidelity, %	(Amp^r + Cam^r)/Amp^r	%	Relative level
Exp. I
Wild type (J1)	144/6,970	2.1	1.0	100 (4/4)	51/5,270	0.97	1.0
Ku70SKO	267/32,300	0.83	0.4	100 (4/4)	93/9,520	0.98	1.0
Ku70DKO	1/17,510	0.0057	0.0027	0 (0/1)	7/6,800	0.1	0.1
Exp. II
Wild type (J1)	24/9,690	0.25	1.0	100 (6/6)	1,132/251,600	0.45	1.0
Ku70SKO	49/29,920	0.16	0.64	100 (6/6)	104/42,330	0.25	0.55
Ku70DKO	0/13,430	<0.007	<0.02	—	5/41,140	0.012	0.027
Exp. III
Wild type (J1)	136/96,000	0.14	1.0	100 (6/6)	99/29,600	0.45	1.0
Ku70SKO	66/75,200	0.088	0.63	100 (6/6)	47/21,600	0.22	0.67
Ku70DKO	0/5,950	<0.01	<0.07	—	7/206,400	0.0034	0.01
Exp. IV
Wild type (J1)	105/6,720	1.56	1.0	100 (6/6)	262/37,500	0.7	1.0
Ku70SKO	148/17,880	0.83	0.53	100 (6/6)	279/44,040	0.63	0.9
Ku70DKO	2/24,210	0.0083	0.0053	0 (0/2)	3/13,230	0.023	0.03