Skip to main content
. Author manuscript; available in PMC: 2008 Dec 6.
Published in final edited form as: Neuron. 2007 Dec 6;56(5):851–865. doi: 10.1016/j.neuron.2007.10.021

Figure 4.

Figure 4

GABAergic synaptic transmission occurs between hippocampal neurons in high Mg++ (10 mM)/low Ca++ (0.2 mM) Ringer, and is blocked by GAT-1 antagonists. A) Dual patch clamp recordings from a pair of rat hippocampal neurons in culture after pretreatment with vigabatrin (100 μM; 128 hours). Shown are IPSCs in response to high frequency presynaptic stimulation (50 Hz for 9 s). The first trace was recorded in Ringer with 2 mM Ca++. The next three traces were made in high Mg++/low Ca++ Ringer. The IPSCs were blocked by gabazine (400 nM). B) IPSCs could also be obtained in high Mg++/low Ca++ solution from neurons that had not been treated with vigabatrin. C) IPSCs in high Mg++/low Ca++ Ringer were blocked by NO-711 (10 μM). Bars in A–C indicate time of presynaptic stimulation. D) Concentration response curve for NO-711. Each data point represents 7–9 different neuron pairs in high Mg++/low Ca++ Ringer. Solid line is a logistic fit (Microcal Origin). IC50 was 0.43 μM. E) Concentration/response curve for SFK-89976a. Each data point represents 8–13 different neuron pairs in high Mg++/low Ca++ Ringer. IC50 was 1.3 μM.