Figure 4.

In vitro cytotoxicity of splenic T cells isolated from CD158b transgenic mice. Freshly isolated nonadherent splenocytes from CD158b transgenic mice (Tg CD158b, L26 mouse line) and nontransgenic littermates (non Tg) were tested in a redirected killing assay against P815 target cells at an effector:target ratio of 100:1. Anti-CD3 mAb-induced cytotoxicity was inhibited in Tg CD158b T cells upon CD158b engagement by HLA-Cw3 expressed on target cells (A) or by anti-CD158b mAb (B). Neither HLA-Cw3 expression (A) nor anti-CD158b mAb (B) could induce inhibition of anti-CD3 redirected target cell lysis by non-Tg T cells. The following mice were used in this representative experiment: L26.4 (H-2^b/b) and L26.5 (H-2^k/b).