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Immunoadsorbed myosin V from interphase (A) or metaphase (B) extracts is able to transport Sepharose beads in the Nitella motility assay. The beads appear as large, refractile spheres, whereas the smaller oval objects are the underlying Nitella chloroplasts. The centers of three beads in each panel were tracked over 30 min; each white dot overlaid on the video frames represents the position of the beads in 1 min increments. Bar, 20 μm.
