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. 1999 Sep 20;146(6):1211–1226. doi: 10.1083/jcb.146.6.1211

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Hybridization of DNA from the H3 isochore fraction to human metaphase spreads. FISH with DNA from the H3 isochore fraction as a probe (FITC-detected) was performed on metaphase spreads from male human lymphocytes. Chromosomes were DAPI-banded and arranged into standard karyograms. The inverted DAPI image (a) displays G- and C-bands more darkly stained compared with R-bands. Most of the R-bands hybridized specifically to the DNA probe as the inverted image of the hybridization signals shows (b, FITC fluorescence appears dark) that displays a typical R banding pattern. Note the different signal intensities of distinct R-bands (e.g., on the distal p-arm of chromosome 1 and the q-arm of chromosome 13).