Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Sep 20;146(6):1211–1226. doi: 10.1083/jcb.146.6.1211

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

R-band DNA is confined to the interior compartment during interphase. Nuclei (a–c, HeLa S6 nucleus; and d–f, three different nuclei from SH-EP N14 cells) were replication-labeled with Cy3-dUTP (replication patterns depicted in red). DNA from the H3 isochore fraction (FITC-detected, depicted in green) was hybridized to Cy3-labeled nuclei. Nuclei were analyzed by confocal microscopy. For each nucleus, identical midnuclear planes are shown regarding FITC or Cy3 fluorescence detection (colocalizing FITC and Cy3 fluorescence appears yellow on merged [b and d–f] images). Regarding the HeLa nucleus, the merge (b) of the hybridization signal (a) and the type I replication pattern (c) reveals the localization of R-band sequences within the interior compartment. A similar localization of R-band sequences is obvious regarding SH-EP N14 cells (d, underlying type I pattern in red). R-band sequences are excluded from the peripheral (e, red, type III pattern) and late replicating (f, red, type V pattern) compartments. It should be noted that all confocal images presented were not further processed and reflect the resolution limits of light microscopy. Therefore, some colocalization of fluorescent signals at the boundaries of differently labeled distinct compartments is expected.