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. 1999 Sep 20;146(6):1319–1332. doi: 10.1083/jcb.146.6.1319

Figure 2.

Figure 2

Arp2/3 complex is essential for actin nucleation at the surface of E. coli (IcsA) and Listeria. a, Arp2/3 complex depletion in platelet extracts. Increasing amounts (20, 40, and 80 μg) of glutathione Sepharose-coupled GST or GST-VCA beads were incubated with 25 μl of platelet extracts. Arp2/3 depletion is monitored by immunoblotting using anti-Arp3 polyclonal antibody. 1, Arp3 in untreated platelet extracts; 2–4, Arp2/3-depleted extracts; 5–7, mock-depleted extracts; 8–10, Arp3 associated with GST-VCA beads; and 11–13, Arp3 associated with GST beads. b, VCA affinity-purified Arp2/3 complex from bovine brain. The composition of the Arp2/3 complex is shown. The seven polypeptides are detected by Coomassie blue staining after SDS-PAGE. c, In vitro reconstitution of actin filaments assembly on E. coli (IcsA) and Listeria surface. N-WASP–coated E. coli (IcsA) or Listeria were incubated in a solution of pure rhodamine-labeled F-actin (10 μM) with or without 0.15 μM pure Arp2/3 complex as indicated. Left panels, phase-contrast; right panels, fluorescence. Bar, 5 μm.