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. 1999 Aug 23;146(4):831–842. doi: 10.1083/jcb.146.4.831

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of the fimbrin–vimentin complex in polarized IC-21 cells. 3 h after attachment, the cells show, by phase microscopy, an asymmetrical morphology (A). Using epifluorescence microscopy the fimbrin–vimentin complex is shown to be restricted to foci present at the cells leading edge where the lamella is actively expanding (D and E). The vimentin network is extended out in the direction of the leading edge (C). The fimbrin–vimentin complex is also detected in retraction fibers at the trailing end of the cell (F and G).