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. 1999 Aug 23;146(4):777–790. doi: 10.1083/jcb.146.4.777

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Sid2p-GFP localization depends on the other Sid proteins. (A) sid1-239 (YDM420) and cdc11-123 (YDM421) cells expressing Sidp2-GFP were grown to mid-log phase at 25°C and then shifted to 36°C for 2.5 h, and fixed and stained for DNA. (B) Sid2p-GFP protein is stable in the sid mutants. Cells expressing no Sid2p-GFP (Wild-type; YDM105), or expressing Sid2p-GFP in a wild-type background (sid2-GFP; YDM415) or in sid4-A1 (sid2-GFP, sid4-A1; YDM419), sid1-239 (sid2-GFP, sid1-239; YDM420), cdc11-123 (sid2-GFP, cdc11-123; YDM421), or cdc7-24 (sid2-GFP, cdc11-123; YDM422) mutant background were grown as described in A, and then Sid2p-GFP levels were analyzed by Western blotting with antibodies against GFP.