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. 1999 Aug 23;146(4):777–790. doi: 10.1083/jcb.146.4.777

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Sid2p has protein kinase activity which is essential for its function. (A) Immune complex kinase assays were performed using myc antibodies (see Materials and Methods) on lysates from wild-type cells, or cells expressing Sid2p-13Myc, HA-Sid2, or HA-Sid2-K238R. Assays were carried out with (+) or without (−) addition of MBP as an artificial substrate. (B) sid2-250 cells (YDM71) transformed with plasmids pRep42, pRep42-GFPsid2, or pRep42-GFPsid2-K238 were plated at 25°C or 36°C on plates containing thiamine.