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. 1999 Aug 23;146(4):777–790. doi: 10.1083/jcb.146.4.777

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Sid2p kinase activity is cell cycle regulated. cdc25-22 sid2-13Myc cells (YDM500) were blocked at 36°C for 3 h then returned to 25°C, allowing them to synchronously enter the cell cycle. Every 15 min, samples were collected for measurement of kinase activity as well as the percentage of cells that were in anaphase/telophase (circles) and the percentage undergoing septation (open diamonds). Sid2p kinase activity was plotted (filled diamonds) by first normalizing the amount of MBP phosphorylation (bottom panel) to the amount of Sid2-13Myc protein present as determined by Western blotting.