Figure 3.

Adenylate kinase and sulfite oxidase loss from mitochondria, like loss of cytochrome c, precedes DEVDase activation. Xenopus mitochondria were incubated in either Xenopus cytosol or buffer, at either 0 or 22°C for 4 h. At the times indicated, 100-μl aliquots were centrifuged and the mitochondrial pellets washed twice, lysed in 1% Triton X-100, then assayed for adenylate kinase activity (A). Data are means and SD of triplicates. (B) At the times indicated, aliquots (2 μl) were assessed for DEVDase activity to determine the time of cytochrome c release. Data are representative of 6 experiments. (C) Rat liver mitochondria (5 mg/ml) were incubated in Xenopus egg cytosol at either 0 or 22°C for 8 h. At the indicated times, aliquots were assessed for cytochrome c and sulfite oxidase translocation by Western blot. Data are representative of three experiments.