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. 1999 Nov 15;147(4):809–822. doi: 10.1083/jcb.147.4.809

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Outer mitochondrial membrane permeability to cytochrome c is bidirectional in the Xenopus cell-free system. Xenopus mitochondria were incubated in cytosol at 0 or 22°C for 4 h in the presence of Sf-9 cell lysates (2% vol/vol) containing either β-galactosidase or Bcl-2 expressed after baculovirus infection. At the times indicated, 2-μl aliquots were assayed for either DEVDase activity (top) or complex IV accessibility (bottom).