Figure 4.

Localization of YAP65, ezrin, and EBP50 in primary well differentiated human nasal epithelia. (A) Well differentiated, pseudostratified primary cultures of human nasal epithelia were counterstained with hemotoxylin and eosin and analyzed by confocal microscopy. A higher magnification of the lumenal surface is shown on the right. (B) Primary human nasal epithelial cultures were incubated with antiserum directed against ezrin (1:300) and EBP50 (1:200). (C) Primary human nasal epithelial cultures were incubated with antiserum directed against YAP65 (1:200) and labeled with 70 nM FITC-conjugated phalloidin to visualize actin. (D) Primary human nasal epithelial cultures were incubated with antiserum directed against ezrin (1:300) and YAP65 (1:200). After washes, the cultures (B–D) were incubated with appropriate Texas red– or FITC-conjugated secondary antisera and mounted for confocal analysis. Bar, 10 μm.