Figure 9.

Localization of c-Yes in airway epithelial cells. (A) Well differentiated primary human nasal epithelia and (B)16HBE14o− cells grown to confluence on transwell filters were fixed and blocked as described in Materials and Methods followed by incubation in antisera specific for c-Yes (1:100), YAP65 (1:200), or EBP50 (1:200). After washes, the cultures were incubated with appropriate Texas red– or FITC-conjugated secondary antisera and analyzed on the confocal microscope. Bars, 10 μm.