Figure 6.

The polarized distribution of LexA–Myo2p tail requires functional endogenous MYO2. (A) Immunofluorescence of LexA in ABY551 (MYO2) and ABY553 (myo2-16) bearing pCLM, grown in raffinose medium with 2% galactose added for 60 min at 25°C, then shifted to 34.5°C for 5 min. Arrows indicate small buds lacking LexA staining. Images overrepresent the proportion of polarized cells severalfold. Bar, 5 μm. (B) Percentages of cells showing polarized LexA staining. ABY551 (MYO2⁺) and ABY553 (myo2-16) bearing pCLM or pCL were grown in raffinose medium, then supplemented with 2% galactose for 90 min at 20°C or for 85 min at 20°C, then 34.5°C for 5 min. For each condition, 200 random cells in four separate trials were scored for staining concentrated at presumptive growth sites. After shift to 34.5°C, myo2-16 cells are less often polarized for LexAp staining than wild-type (P ≤ 0.05, two-tailed t test).