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. 1999 Nov 15;147(4):791–808. doi: 10.1083/jcb.147.4.791

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The polarized distribution of LexA–Myo2p tail requires functional SEC2. (A) LexAp, Myo2p, and Sec4p in NY13 (wt, upper panels), NY130 (sec2-41, middle panels), and NY3 (sec1-1, lower panels) bearing pCLM, grown in raffinose, then supplemented with 2% galactose for 60 min at 28°C and then shifted to 35°C for 5 min. Bar, 5 μm. (B) Percentages of cells showing polarized LexAp staining. After shift to 35°C, sec2-41 cells are less often polarized for LexAp staining than wild-type (P ≤ 0.05, two-tailed t test), whereas sec1-1 cells are more often polarized for LexAp staining than wild-type (P ≤ 0.05).